Collagen VI alpha 1 Rabbit Polyclonal Antibody

• Catalog Number: orb5898
• Category: Antibodies
• Description: Collagen VI alpha 1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Collagen VI (951-1028/1028aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 106 kDa
• Target: COL6A1
• UniProt ID: P12111
• RRID: AB_10921246
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: CO6A1_HUMAN; COL6A1; COL6A2; COL6A3; Collagen alph
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Collagen VI alpha 1) Polyclonal Antibody, Unconjugated (orb5898) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Ovary (Mouse) Lysate at 40 ug, Lane 2: Placenta (Mouse) Lysate at 40 ug, Lane 3: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Lane 4: Ovary (Rat) Lysate at 40 ug, Lane 5: U251 (Human) Cell Lysate at 30 ug, Lane 6: U2os (Human) Cell Lysate at 30 ug, Lane 7: SH-SY5Y (Human) Cell Lysate at 30 ug, Primary: Anti-Collagen VI alpha 1 (orb5898) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 109 kD, Observed band size: 125 kD.

Sample: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Primary: Anti-Collagen VI alpha 1 (orb5898) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 106 kD, Observed band size: 131 kD.