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Catalog Number | orb2936 |
---|---|
Category | Antibodies |
Description | COL6A5 Rabbit Polyclonal Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC-Fr, IHC-P |
Predicted Reactivity | Human |
Reactivity | Rat |
Isotype | IgG |
Immunogen | KLH conjugated synthetic peptide derived from human COL6A5/Collagen VI alpha 5 (121-220/2615aa) |
Antibody Type | Primary Antibody |
Concentration | 1mg/ml |
Dilution range | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 288 kDa |
Target | COL6A5 |
UniProt ID | A8TX70 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
Alternative names | Collagen VI alpha 5; CO6A5_HUMAN; COL29A1; Col6a5; Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Tissue/Cell: rat skin tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-COL6A5/Collagen VI alpha 5 Polyclonal Antibody, Unconjugated (orb2936) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
WB | |
Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat | |
Rabbit | |
Polyclonal | |
HRP |
WB | |
Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat | |
Rabbit | |
Polyclonal | |
FITC |
WB | |
Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat | |
Rabbit | |
Polyclonal | |
Biotin |