Collagen IV antibody

• Catalog Number: orb2276391
• Category: Antibodies
• Description: Collagen IV antibody validated for immunohistochemistry on 76 different Normal Tissues
• Clonality: Monoclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Purified human placental extract
• UniProt ID: P02462, P53420, P08572, P29400
• Tested applications: IHC
• Dilution range: 1:100-1:200
• Application notes: Positive Control: Colon: An at least moderate collagen IV immunostaining of basement membranes and a strong staining of vessels and the tunica muscularis mucosa should be seen. Negative Control: Colon: Collagen IV immunostaining must be absent in epithelial cells. Cellular Localization: Cytoplasmic and extracellular Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
• Clone Number: Biorbyt-704R
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Arresten; BSVD; COL4A1; COL4A1 NC1 domain; COL4A2;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Collagen IV positive membranes surround pancreatic acini. Small blood vessels do also stain positive.

In the intestine collagen IV immunostaining involves basement membranes vessels of all sizes and smooth muscle cells of the lamina muscularis mucosae.

Papillary renal cell carcinoma showing a delicate collagen IV positive membrane between tumor cells and the stroma and an intense staining of vessels.