Cisplatin

SKU: orb1308163

Description

Cisplatin is a classic DNA-crosslinking chemotherapeutic agent that induces cell death via DNA adduct formation, autophagy, and ferroptosis activation. It is widely used in oncology research and for establishing in vivo models of acute and chronic kidney injury.

Research Area

Cell Biology, Molecular Biology

Key Properties

• CAS Number: 15663-27-1
• MW: 300.04
• Purity: 99.63%
• Formula: Cl₂H₆N₂Pt
• SMILES: N[Pt](Cl)(Cl)N
• Target: DNA Alkylator/Crosslinker,Ferroptosis,Autophagy,DNA/RNA Synthesis
• Solubility: H2O:1 mg/mL (3.3 mM);DMF:20 mg/mL (66.66 mM)

Bioactivity

• Target IC50:
  A549 cells:1.58 µM|MCF-7 cells:1.2 µM|SK-MES-1 cells:4.09 µM|HCC38 cells:2.5 µM
• In Vivo:
  METHODS: To assay anti-tumor activity in vivo, Cisplatin (5 mg/kg/6 days) and Chloroquine (13 mg/kg/day) were intraperitoneally injected into BALB/c nude mice bearing hypopharyngeal squamous cell carcinoma tumors (HSCC) FaDu for eighteen days. RESULTS: Cisplatin treatment significantly inhibited HSCC tumor growth. Chloroquine inhibited autophagy and increased Cisplatin-induced apoptosis, which enhanced the efficacy of Cisplatin, resulting in reduced tumor growth and prolonged survival in mice. METHODS: To attenuate nephrotoxicity induced by Cisplatin treatment, Cisplatin (3-6 mg/kg/3 days) was administered intraperitoneally and Cilastatin (100 mg/kg/day) subcutaneously to BALB/c mice with human lung adenocarcinoma tumor A549 for seven days. RESULTS: Cilastatin can reduce the induced nephrotoxicity of Cisplatin without affecting its antitumor effects.
• In Vitro:
  METHODS: Human non-small cell lung cancer cells A549, SKMES-1, MOR and H460 were treated with Cisplatin (0.001-100 μM) for 72 h, and cell growth inhibition was detected by MTT. RESULTS: Cisplatin dose-dependently inhibited the cell growth of A549, SKMES-1, MOR and H460 cells with IC50 of 1.58 µM, 4.09 µM, 6.39 µM and 5.72 µM, respectively. METHODS: Human breast cancer MCF-7 and MDA-MB-231 were treated with Cisplatin (2-10 μg/mL) for 48 h, and the expression levels of target proteins were detected by Western Blot. RESULTS: Cisplatin dose-dependently induced increased levels of apoptosis-related proteins cleaved-caspase 3 and cleaved-PARP in MCF-7 and MDA-MB-231 cells. METHODS: Mesothelioma cells JU77, LO68 and ONE58 were treated with Cisplatin (5-100 μg/mL) for 24 h, and mitochondrial membrane potential (MMP) was detected using JC-1 dye. RESULTS: Cisplatin dose-dependently decreased MMP and inhibited mitochondrial function in JU77, LO68 and ONE58 cells.
• Cell Research:
  Rabbit renal proximal tubules were isolated using the iron oxide perfusion method and grown in 35-mm tissue culture dishes under improved conditions as described previously. The cell culture medium was a 1:1 mixture of Dulbecco's modified Eagle's medium/Ham's F-12 (without D-glucose, phenol red, or sodium pyruvate) supplemented with 15 mM HEPES buffer, 2.5 mM L-glutamine, 1 μM pyridoxine HCl, 15 mM sodium bicarbonate, and 6 mM lactate. Hydrocortisone (50 nM), selenium (5 ng/ml), human transferrin (5 μg/ml), bovine insulin (10 nM), and L-ascorbic acid-2-phosphate (50 μM) were added to fresh culture medium immediately before daily media change. In general, confluent RPTCs were treated with inhibitors or diluent control [typically DMSO at 0.1% (v/v)] for 30 min before treatment with cisplatin. Aliquots of RPTCs were used for various assays as detailed below .
• Animal Research:
  Mice were divided randomly into three groups (Control, Cisplatin and Cisplatin+HemoHIM), and each group consisted of twenty mice. B16F0 melanoma (5 × 10^5 cells/mouse) was inoculated into subcutaneous femoral left region of mice at 3 days before an initial injection of cisplatin. Cisplatin was injected intraperitoneally at 4 mg/kg body weight (B.W.) on day 0, 7 and 14 (total three injections). Experimental group was intubated with HemoHIM at a final concentration of 100 mg/kgB.W. by everyday from day -1 to day 16, while the control group received only water. On day 17 after initial injection of cisplatin, all mice of each group were experimented, respectively, to evaluate tumor weight or tumor size. The tumor size was calculated as follows: tumor size = ab^2/2, where a and b are the larger and smaller diameters, respectively .

Storage & Handling

• Storage: keep away from direct sunlight | Powder: -20°C for 3 years | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

damage, Crosslinker, cross-linking, drug, DNASynthesis, DNA synthesis, DNA Alkylator/Crosslinker, DNA Alkylator, DNA, DNAAlkylator, DNA Synthesis, chemotherapy, CDDP, Cisplatin, cis-Platinum, cis-Diaminodichloroplatinum, Autophagy, antineoplastic, inhibit, Inhibitor, Ferroptosis, RNA Synthesis, RNASynthesis

Compound Identifiers

PubChem CID

2767