CHEK2 Antibody (N-term)

• Catalog Number: orb1930721
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB19067
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Dilution range: IF: 1:10~50, WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 60915 Da
• Target: This CHEK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-141 amino acids from the N-terminal region of human CHEK2.
• UniProt ID: O96017
• NCBI: NP_001005735.1, NP_665861.1, NP_001244316.1, NP_009125.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Serine/threonine-protein kinase Chk2, CHK2 checkpo
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

CHEK2 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of CHEK2 Antibody (N-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

CHEK2 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CHEK2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

CHEK2 Antibody (N-term) western blot analysis in K562, NCI-H460 cell line lysates (35 ug/lane). This demonstrates the CHEK2 antibody detected the CHEK2 protein (arrow).

All lanes: Anti-CHEK2 Antibody (N-term) at 1:1000 dilution. Lane 1: HCT116 whole cell lysate. Lane 2: Jurkat whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 61 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.