CELA3B Antibody / Elastase 3B

• Catalog Number: orb699545
• Category: Antibodies
• Description: This MAb recognizes a protein of ~30kDa, identified as CELA3B (Chymotrypsin like elastase family member 3B). Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein; it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CELA3B/1374
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: Mouse IgG3, kappa
• Immunogen: A portion of amino acids 82-238 from the human protein was used as the immunogen for the CELA3B antibody.
• Dilution range: Western blot: 1-2ug/ml,ELISA: 2-4ug/ml; order Ab without BSA for coating
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This CELA3B antibody is available for research use only.
• UniProt ID: P08861
• Storage: Store the CELA3B antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the CELA3B antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.