CDDO-Im

SKU: orb1296189

Description

CDDO-Im

Research Area

Cell Biology, Immunology & Inflammation, Metabolism Research, Molecular Biology

Key Properties

• CAS Number: 443104-02-7
• MW: 541.72
• Purity: 98.30% (May vary between batches)
• Formula: C₃₄H₄₃N₃O₃
• SMILES: [H][C@@]12CC(C)(C)CC[C@@]1(CC[C@]1(C)[C@]2([H])C(=O)C=C2[C@@]1(C)CC[C@@]1([H])C(C)(C)C(=O)C(=C[C@]21C)C#N)C(=O)n1ccnc1
• Target: Ferroptosis|||PPAR|||Nrf2
• Solubility: H2O:Insoluble;DMSO:40 mg/mL (73.84 mM);10% DMSO+90% Corn Oil:2 mg/mL (3.69 mM)

Bioactivity

• Target IC50:
  PPARα:232 nM (Ki)|PPARγ:344 nM (Ki)
• In Vivo:
  CDDO-Im exhibits strong inhibition of de novo inducible nitric oxide synthase (iNOS) expression in primary mouse macrophages. Additionally, it effectively suppresses the proliferation of B16 murine melanoma and L1210 murine leukemia cells in vivo. Administering 10 nM (5.4 μg) of CDDO-Im significantly impedes the induction of iNOS by IFN-γ, while a dose as low as 1 nmol (0.54 μg) of CDDO-Im shows partial effectiveness .
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• In Vitro:
  CDDO-Im effectively suppresses the proliferation of human leukemia and breast cancer cell lines (IC50 approximately 10-30 nM). In U937 leukemia cells, it induces monocytic differentiation, evidenced by increased CD11b and CD36 expression . CDDO-Im treatment causes G2/M-phase cell cycle arrest and apoptosis in triple-negative breast cancer cell lines SUM159 and MDA-MB-231, significantly inhibiting CD24−/EpCAM+ cells in SUM159 tumorspheres. It also decreases sphere-forming efficiency and size in both primary and secondary cultures . Moreover, CDDO-Im elevates Nrf2 protein levels, which increases the expression of antioxidant and detoxification genes regulated by Nrf2 .
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• Cell Research:
  CDDO-Im is dissolved in DMSO. SUM159 and MDA-MB-231 cells are seeded into each well of 96-well plates (1,000 cell/well) and treated the next day with vehicle control or CDDO-Im (1, 10, 50, 100 and 200 nM) for given incubation time. The absorbance is measured with a spectrophotometer to determine the cell proliferation rate .
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• Animal Research:
  Mice are injected i.p. with thioglycollate, and the resulting resident peritoneal macrophages are activated 3 days later with an i.p. injection of IFN-γ. CDDO and CDDO-Im are injected i.p. 30 min after IFN-γ. Macrophages are harvested 10 h later, cultured for 12 h, and then assayed for expression of iNOS protein and production of nitric oxide (NO) .
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CDDO-Imidazolide, CDDOIm, CDDO-Im, CDDO Im, Keap1-Nrf2, Nrf2, inhibit, Ferroptosis, Inhibitor, Peroxisome proliferator-activated receptors, PPARγ, PPARα, PPAR, TP 235, TP235, TP-235, RTA403, RTA-403, RTA 403

Compound Identifiers

PubChem CID

9958995