CD62L Recombinant Rabbit Monoclonal Antibody

SKU: orb1172983

Description

CD62L Recombinant Rabbit Monoclonal Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FC, ICC, WB
• Dilution Range: WB=1:500-1000, ICC/IF=1:100-500, Flow-Cyt=1:50
• Reactivity: Human
• Predicted Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: G3B6
• Target: SELL
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD62L; L-selectin; LAM-1; LECAM-1; LECAM1; Lnhr; Ly-22; Ly-m22; Lyam-1; Lyam1; LYAM1_MOUSE; Sell; CD62 antigen-like family member L; Leukocyte adhesion molecule 1 (LAM-1); Leukocyte-endothelial cell adhesion molecule 1 (LECAM1); Lymph node homing receptor; Lymphocyte antigen 22 (Ly-22); Lymphocyte surface MEL-14 antigen; Ly22; LAM1; LEU8; LSEL; PLNHR; TQ1; LYAM1_HUMAN; Leukocyte surface antigen Leu-8; gp90-MEL;

Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: Human serum, Protein loading quantity: 20 µg, Exposure time: 30 s, Predicted MW: 42 kDa, Observed MW: 100 kDa.

Cell line: EL4, Fixation: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary Ab Dilution: 1:50, Primary Ab incubation condition: 4°C overnight, Secondary Ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1172983.

Cell line: Jurkat, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4°C overnight, Secondary ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1172983.

Cell line: PBMC, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Secondary ab: Goat anti Rabbit IgG, Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line). Isotype control: Rabbit monoclonal IgG (Blue line). Comment: Line red is the positive signal for orb1172983.

Cell line: Raji, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4°C overnight, Secondary ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1172983.

Cell line: Ramos, Fixation: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary Ab Dilution: 1:50, Primary Ab incubation condition: 4°C overnight, Secondary Ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1172983.

Quick Database Links

Gene Symbol

SELL