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CD45 Antibody (Leukocyte marker)

Catalog Number: orb749435

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb749435
CategoryAntibodies
DescriptionCD45, also referred to as CD45R and PTPRC (Protein tyrosine phosphatase receptor type C), has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Along with other members of the PTP family, it regulates a number of cellular processes including cell differentiation, growth and mitotic cycle, and is an essential regulator of B- and T-cell antigen receptor-mediated activation.Multiple isoforms of CD45 are distributed throughout the immune system and arise due to alternative splicing of exons located in the N-terminus. CD45RA contains the A exon and is a naive T-cell marker which may help prevent autoimmune disease. CD45RB contains B and stains most leukemias and lymphomas. CD45RC contains C and stains thymocytes, monocytes and dendritic cells. CD45RO doesn't contain A, B or C and is a marker of activated T-cells that can be used to classify and diagnose and classify lymphomas. This antibody will bind to all CD45 isoforms. The variation in these isoforms is localized to the extracellular domain, with the intracellular domain being conserved. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.
Species/HostMouse
ClonalityMonoclonal
Clone Number135-4C5
Tested applicationsFACS, IF, IHC-P
ReactivityHuman
IsotypeMouse IgG2b, kappa
ImmunogenStimulated human leukocytes were used as the immunogen for the CD45 antibody.
Antibody TypePrimary Antibody
Dilution rangeFlow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
PurityProtein G affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Hazard InformationThis CD45 antibody is available for research use only.
UniProt IDP08575
StorageCD45 antibody (with azide) can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
NoteFor research use only
Application notesDue to variation in protocol and secondary antibody used, the CD45 antibody may need to be titered for optimal performance.1. FFPE staining requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Expiration Date12 months from date of receipt.
CD45 Antibody (Leukocyte marker)

Immunofluorescent staining of PFA-fixed human Jurkat cells with CD45 antibody (clone 135-4C5, green) and Reddot nuclear stain (red).

CD45 Antibody (Leukocyte marker)

IHC staining of FFPE human tonsil tissue with CD45 antibody (clone 135-4C5).

CD45 Antibody (Leukocyte marker)

Flow cytometry testing of lymphocyte-gated human PBM cells with CD45 antibody (clone 135-4C5, green), and unstained cells (gray).

CD45 Antibody (Leukocyte marker)

Flow cytometry staining of PFA-fixed human Jukat cells with CD45 antibody (clone 135-4C5); Red = isotype control, Blue = CD45 antibody.

CD45 Antibody (Leukocyte marker)

SDS-PAGE Analysis of Purified, BSA-Free CD45 Antibody (clone 135-4C5). Confirmation of Integrity and Purity of the Antibody.

  • CD45 (Intracellular) [orb1294366]

    FC,  IF,  IHC,  WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    25 μl, 100 μl
  • CD45 (Extracellular) [orb1294367]

    FC,  IF,  IHC,  WB

    Human, Mouse

    Rabbit

    Polyclonal

    Unconjugated

    25 μl, 100 μl
  • CD45 Rabbit Polyclonal Antibody [orb1179]

    FC

    Mouse, Rat

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 200 μl, 50 μl
  • CD45 Rabbit Polyclonal Antibody [orb312177]

    ICC,  IF,  IHC-Fr,  IHC-P,  WB

    Bovine

    Human

    Rabbit

    Polyclonal

    Unconjugated

    200 μl, 100 μl, 50 μl
  • CD45RA Antibody (Leukocyte marker) [orb248519]

    FACS,  IF,  IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    20 μg