CD45 Antibody (Leukocyte marker)

• Catalog Number: orb699518
• Category: Antibodies
• Description: CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PTPRC/1666
• Tested applications: FACS, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Human recombinant full-length protein was used as the immunogen for the CD45 antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Western blot: 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This CD45 antibody is available for research use only.
• UniProt ID: P08575
• Storage: Store the CD45 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the CD45 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Flow cytometry testing of PFA-fixed human Jurkat cells with CD45 antibody (clone PTPRC/1666); Red=isotype control, Blue= CD45 antibody.

Western blot testing of human spleen lysate with CD45 antibody. Expected molecular weight: 147-220 kDa depending on glycosylation level.