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CD45 Antibody (Leukocyte marker)

Catalog Number: orb699518

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb699518
CategoryAntibodies
DescriptionCD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.
Species/HostMouse
ClonalityMonoclonal
Clone NumberPTPRC/1666
Tested applicationsFACS, WB
ReactivityHuman
IsotypeMouse IgG1, kappa
ImmunogenHuman recombinant full-length protein was used as the immunogen for the CD45 antibody.
Antibody TypePrimary Antibody
Dilution rangeFlow cytometry: 1-2ug/million cells,Western blot: 1-2ug/ml
PurityProtein G affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Hazard InformationThis CD45 antibody is available for research use only.
UniProt IDP08575
StorageStore the CD45 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
NoteFor research use only
Application notesOptimal dilution of the CD45 antibody should be determined by the researcher.
Expiration Date12 months from date of receipt.
CD45 Antibody (Leukocyte marker)

Flow cytometry testing of PFA-fixed human Jurkat cells with CD45 antibody (clone PTPRC/1666); Red=isotype control, Blue= CD45 antibody.

CD45 Antibody (Leukocyte marker)

Western blot testing of human spleen lysate with CD45 antibody. Expected molecular weight: 147-220 kDa depending on glycosylation level.

  • CD45 (Intracellular) [orb1294366]

    FC,  IF,  IHC,  WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    25 μl, 100 μl
  • CD45 (Extracellular) [orb1294367]

    FC,  IF,  IHC,  WB

    Human, Mouse

    Rabbit

    Polyclonal

    Unconjugated

    25 μl, 100 μl
  • CD45 Rabbit Polyclonal Antibody [orb1179]

    FC

    Mouse, Rat

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 200 μl, 50 μl
  • CD45 Rabbit Polyclonal Antibody [orb312177]

    ICC,  IF,  IHC-Fr,  IHC-P,  WB

    Bovine

    Human

    Rabbit

    Polyclonal

    Unconjugated

    200 μl, 100 μl, 50 μl
  • CD45RA Antibody (Leukocyte marker) [orb248519]

    FACS,  IF,  IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    20 μg