CD45 Antibody (Leukocyte marker)

• Catalog Number: orb699518
• Category: Antibodies
• Description: CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Human recombinant full-length protein was used as the immunogen for the CD45 antibody.
• UniProt ID: P08575
• Tested applications: FACS, WB
• Dilution range: Flow cytometry: 1-2ug/million cells,Western blot: 1-2ug/ml
• Application notes: Optimal dilution of the CD45 antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: PTPRC/1666
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This CD45 antibody is available for research use only.
• Note: For research use only

Flow cytometry testing of PFA-fixed human Jurkat cells with CD45 antibody (clone PTPRC/1666); Red=isotype control, Blue= CD45 antibody.

Western blot testing of human spleen lysate with CD45 antibody. Expected molecular weight: 147-220 kDa depending on glycosylation level.