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CD272 [BTLA] Recombinant Protein
Description
Research Area
Images & Validation
−![CD272 [BTLA] Recombinant Protein](/images/quality_badge_proteins.png)
| Application Notes |
|---|
Key Properties
−| Source | CHO cells |
|---|---|
| Biological Activity | Measured in a competitive binding assay. |
| Target | BTLA |
| Tag | Fc Tag |
| Protein Sequence | The extracellular domain of human CD272 (aa 31-157) is fused to the N-terminus of the Fc region of mouse IgG2a. |
| Purity | >98% (SDS-PAGE). Endotoxin level is less than 0.06EU/ μg protein (LAL test; Lonza). |
Storage & Handling
−| Storage | Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Lyophilized from 0.2um-filtered solution in PBS. Reconstitute with 100 μl sterile water. Add 1X PBS to the desired protein concentration. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Human BTLA [orb2994015]
Unconjugated
SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE.
Predicted: 40.9 KDa. Observed: 55 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgHuman BTLA [orb2993930]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 40.4 KDa. Observed: 50-70 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgHuman BTLA [orb2994992]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 14.79 KDa. Observed: 30 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgHuman BTLA Protein, mFc-His Tag [orb689405]
Unconjugated
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 41.3 kDa after removal of the signal peptide.
Mammalian
10 μg, 100 μg, 50 μgHuman BTLA Protein, hFc Tag [orb757466]
Unconjugated
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 39.9 kDa after removal of the signal peptide.The apparent molecular mass of Human-BTLA-hFc is approximately 55-70 kDa due to glycosylation.
Mammalian
100 μg, 10 μg, 50 μg

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Protocol Information
CD272 [BTLA] Recombinant Protein (orb1229992)
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