CD166 Antibody (N-term)

• Catalog Number: orb1931368
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This CD166 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58-87 amino acids from the N-terminal region of human CD166.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse, Rabbit, Rat
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• UniProt ID: Q13740
• MW: 65102 Da
• Tested applications: FC, IHC-P, WB
• Dilution range: WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50
• Antibody Type: Primary Antibody
• Clone Number: RB19989
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: CD166 antigen, Activated leukocyte cell adhesion m
• Note: For research use only
• NCBI: NP_001230209.1, NP_001618.2

CD166 Antibody (N-term) flow cytometric analysis of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human brain tissue reacted with CD166 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of CD166 antibody (N-term) in NCI-H460 cell line lysates (35 ug/lane). CD166 (arrow) was detected using the purified Pab.