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Description
Research Area
Images & Validation
−| Tested Applications | WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Human M-CSF, His Tag at 5μg/mL (100 μL/well) can bind Human M-CSF R, Fc Tag with a linear range of 0.17-1.52 ng/mL. |
| Target | CSF1R |
| Tag | C-Fc Tag |
| MW | 80.7 kDa |
| Purity | >95% as determined by SDS-PAGE. |
| Protein Sequence | Ile 20 - Glu 512 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | PBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Recombinant Human M-CSF R Protein [orb2994192]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 81.9 KDa. Observed: 115-130 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Human M-CSF R Protein [orb2994266]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 55.9 KDa. Observed: 80-110 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Cynomolgus CSF1R Protein [orb2994841]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 82.2 KDa. Observed: 105-130 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Cynomolgus CSF1R Protein [orb2994843]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 56.1 KDa. Observed: 80-120 KDa, reducing conditions
1 mg, 10 μg, 50 μg, 500 μgRecombinant Human CD115/CSF1R Protein, C-His [orb2969434]
ELISA, SDS-PAGE, WB
>95% as determined by SDS-PAGE.
57.37 kDa
1 mg, 100 μg, 50 μg

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The purity of rh CSF1R Fc Chimera was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_005202.2 |
|---|
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Protocol Information
CD115 Recombinant Protein (orb1228099)
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