CCL3 Antibody

SKU: orb1247690

Description

CCL3 Antibody

Research Area

Cancer Biology, Infectious Disease & Virology

Images & Validation

• Tested Applications: ELISA
• Predicted Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave bands at approx 26+28kDa (doublet) and 40kDa in Human Bone Marrow and Spleen lysates after 0.1µg/mL antibody staining.The 26+28kDa doublet was also found in Human Lymph Node and in Tonsil lysates. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 10.2kDa according to NP_066286.1. The detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain these bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-EWVQKYVSDLELSA
• Target: CCL3L1; CCL3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CCL3, chemokine (C-C motif) ligand 3, G0S19-1, LD78ALPHA, MIP-1-alpha, MIP1A, SCYA3, LD78 alpha beta, Small inducible cytokine A3, small inducible cytokine A3 (homologous to mouse Mip-1a), CCL3L1, LD78, 464.2, SCYA3L, G0S19-2, SCYA3L1, D17S1718, LD78BETA, MGC12815, chemokine (C-C motif) ligand 3-like 1, small inducible cytokine A3-like 1, MIP-1aP, MGC104178, MGC182017, MIP1AP

Quick Database Links

Gene Symbol

CCL3L1; CCL3

UniProt

P10147

RefSeq (Protein):NP_066286.1, NP_002974.1