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Catalog Number | orb1247690 |
---|---|
Category | Antibodies |
Description | CCL3 Antibody |
Target | CCL3L1; CCL3 |
Clonality | Polyclonal |
Conjugation | Unconjugated |
Predicted Reactivity | Human |
Form/Appearance | Liquid |
Concentration | 500 ug/mL |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Immunogen | The immunogen for this antibody is: C-EWVQKYVSDLELSA |
UniProt ID | P10147 |
Tested applications | ELISA |
Application notes | Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave bands at approx 26+28kDa (doublet) and 40kDa in Human Bone Marrow and Spleen lysates after 0.1ug/ml antibody staining.The 26+28kDa doublet was also found in Human Lymph Node and in Tonsil lysates. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 10.2kDa according to NP_066286.1. The detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain these bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? |
Antibody Type | Primary Antibody |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | CCL3, chemokine (C-C motif) ligand 3, G0S19-1, LD7 Read more... |
Note | For research use only |
NCBI | NP_002974.1, NP_066286.1 |
Expiration Date | 12 months from date of receipt. |
ELISA, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IF, IHC-Fr, IHC-P | |
Human, Mouse | |
Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |