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Cattle Interleukin 1 Receptor Antagonist (IL1RA) ELISA Kit

Catalog Number: orb779745

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb779745
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Receptor Antagonist(IL1RA).
ReactivityBovine
Concentration2000 pg/mL
UniProt IDO77482
Tested applicationsELISA
Application notesstandard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle IL1RA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle IL1RA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle IL1RA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle IL1RA in the samples is then determined by comparing the OD of the samples to the standard curve
Assay TypeSandwich
Assay Time3.5h
Range31.25-2000 pg/mL
Sensitivity11.6 pg/mL
Sample Typesserum, plasma, tissue homogenates and other biological fluids
Alternative namesIL1RN ELISA Kit, IL1-RA ELISA Kit, ICIL1RA ELISA K
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NoteFor research use only
Cattle Interleukin 1 Receptor Antagonist (IL1RA) ELISA Kit