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| Catalog Number | orb779667 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiotensin I Converting Enzyme(ACE). |
| Reactivity | Bovine |
| Concentration | 400 ng/mL |
| UniProt ID | P12820 |
| Tested applications | ELISA |
| Application notes | standard: 400 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle ACE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle ACE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle ACE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle ACE in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Type | Sandwich |
| Assay Time | 3.5h |
| Range | 6.25-400 ng/mL |
| Sensitivity | 2.11 ng/mL |
| Sample Types | Serum, plasma and other biological fluids. |
| Alternative names | CD143 ELISA Kit, ACE1 ELISA Kit, DCP1 ELISA Kit, A Read more... |
| Note | For research use only |
