Carboxylesterase 1/CES1 Antibody

SKU: orb1537788

Description

Carboxylesterase 1/CES1 Antibody

Images & Validation

• Tested Applications: ELISA, IHC, IHC-P, WB
• Dilution range: IHC, IHC-P (2.5 µg/ml), Peptide-ELISA (1:8000), WB (0.03 - 0.1 µg/ml)
• Reactivity: Human
• Application Notes:
  Further information: Immunohistochemistry: LS-B2401 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2401 was determined to be 2.5 ug/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-NTQAAQKLKDKE, from the internal region (near C-Terminus) of the protein sequence according to NP_001020366.1NP_001020365.1NP_001257.4.
• Target: Carboxylesterase 1 / CES1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Concentration: 0.5 mg/ml
• Disclaimer: For research use only

Alternative Names

CES1, ACAT, Brain carboxylesterase hBr1, Carboxylesterase 1, Carboxylesterase 2 (liver), CEH, CES1A1, CES1A2, Cocaine carboxylesterase, Egasyn, HMSE, Liver carboxylesterase 1, HCE-1, PCE-1, Retinyl ester hydrolase, TGH, Triacylglycerol hydrolase, REH, SES1, CE-1, Cholesteryl ester hydrolase, HMSE1, Serine esterase 1

Antibody (0.03 ug/ml) staining of Human Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

Carboxylesterase 1 / CES1