Carbonic Anhydrase II Antibody Peroxidase Conjugated

SKU: orb344731

Description

Carbonic Anhydrase II antibody (Peroxidase)

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:500 - 1:2,500, IHC: 1:100 - 1:500, WB: 1:250 - 1:1,000
• Reactivity: Human
• Application Notes:
  Anti-Carbonic Anhydrase II Peroxidase Antibody has been tested by Western blot and suitable to be assayed against 1.0 ug of Carbonic Anhydrase II [Human Erythrocytes] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:6,000 of the reconstitution concentration is suggested for this product.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Carbonic Anhydrase II [Human Erythrocytes]
• Purity: Carbonic anhydrase II is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Carbonic Anhydrase II [Human Erythrocytes]. Cross reactivity against Carbonic Anhydrase II from other sources may occur but have not been specifically determined.
• Conjugation: HRP

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Carbonic Anhydrase II Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Carbonic Anhydrase II Antibody, Carbonate dehydratase II antibody, Carbonic anhydrase 2 antibody, Carbonic anhydrase B antibody, Carbonic anhydrase C antibody, Carbonic anhydrase II antibody, Carbonic dehydratase antibody, CAC, CA-II

Western Blot of Rabbit anti-Carbonic Anhydrase II Antibody Peroxidase Conjugated. Lane 1: Carbonic Anhydrase II. Load: 50 ng per lane. Primary antibody: Rabbit anti-Carbonic Anhydrase II Antibody Peroxidase Conjugated at 1:1000 overnight at 4°C. Secondary antibody: n/a. Block: orb348637 for 30 minutes at RT. Predicted/Observed size: 29 kDa, 29 kDa for Carbonic Anhydrase II.

Quick Database Links

UniProt

P00918

RefSeq (Protein):NP_000058.1