Calponin 1 Antibody

• Catalog Number: orb385955
• Category: Antibodies
• Description: Calponin is a calmodulin, F-actin and tropomyosin binding protein which is thought to be involved in the regulation of smooth muscle contraction.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Immunogen: Recombinant human protein was used as the immunogen for the Calponin antibody.
• UniProt ID: P51911
• Tested applications: FACS, IF, IHC-P
• Dilution range: Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Application notes: Titration of the b-Catenin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Antibody Type: Primary Antibody
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This Calponin antibody is available for research use only.
• Note: For research use only

IHC testing of FFPE rat uterus stained with Calponin antibody. HIER: steam sections in 10mM Tris with 1mM EDTA, pH9.0, for 10-20 min.

IHC testing of FFPE human uterus stained with Calponin antibody. HIER: steam sections in 10mM Tris with 1mM EDTA, pH9.0, for 10-20 min.