Caffeic Acid Phenethyl Ester

SKU: orb1300776

Description

Caffeic Acid Phenethyl Ester (CAPE), a bioactive propolis component, acts as an NF-kappa B inhibitor with antineoplastic and cytoprotective properties. It suppresses pathways like Akt and p70S6K, and research shows it inhibits vascular smooth muscle cell proliferation via p38 MAPK/HIF-1α, making it relevant for oncology, immunology, and cardiovascular disease studies in vitro and in vivo.

Research Area

Cell Biology, Signal Transduction

Key Properties

• CAS Number: 104594-70-9
• MW: 284.31
• Purity: 99.93%
• Formula: C₁₇H₁₆O₄
• SMILES: Oc1ccc(\C=C\C(=O)OCCc2ccccc2)cc1O
• Target: NF-κB,Apoptosis
• Solubility: DMSO:247.5 mg/mL (870.53 mM);Ethanol:28.4 mg/mL (99.89 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (7.03 mM)

Bioactivity

• In Vivo:
  in vivo Caffeic acid phenethyl ester (10 mg/kg, i.p.) inhibit the growth and angiogenesis of Primary tumors in C57BL/6 and BAL-/- mice inoculated with Lewis lung carcinoma, colon carcinoma, and melanoma cells. Caffeic acid phenethyl ester (5, 10, 20 mg/kg) also shows immunomodulatory effects in vivo by decreasing thymus weight and/or cellularity of thymus and spleen.
• In Vitro:
  Caffeic acid phenethyl ester blocks NF-κB activation induced by phorbol ester, ceramide, okadaic acid, and hydrogen peroxide by preventin the translocation o the p65 subunit of NF-κB to the nucleus. In a series of tumor cell lines, Caffeic acid phenethyl ester shows promising ant Proliferative activity with EC50 of 1.76, 3.16, 13.7, and 44.0 μM against murine colon 26-L5, murine B16-BL6 melanoma, human HT-1080 fibrosarcoma and human lung A549 adenocarcinoma cell lines, respectively Caffeic acid phenethyl ester, as a potent antioxidant, exerts its anti-apoptotic effect in cerebellar granule cells by blocking ROS formation and inhibiting caspase activity. Moreover, Caffeic acid phenethyl ester attenuate the pro-inflammatory phenotype of LPS-stimulated HSCs, and LPS-induced sensitization of HSCs to fibrogenic cytokines by inhibiting NF-κB signaling.
• Cell Research:
  Human HT-1080 fibrosarcoma, human lung A549 adenocarcinoma and murine B16-BL6 melanoma cell lines are maintained in EMEM medium supplemented with 10% FCS, 0.1% sodium bicarbonate and 2 mM glutamine. Murine colon 26-L5 carcinoma cell line, On the other hand is maintained in RPMI medium containin the same supplements as in EMEM. These are All highly metastatic cell lines except for A-549 carcinoma. Cellular viability is determined usin the s andard MTT assay. In brief, exponentially growing cells are Harvested and 100 μl of cell suspension containing 2000 cells is plated in 96-well microtiter plates. After 24 h of incubation to allow for cell attachment the cells are treated with varying concentration of test Samples in medium (100 μl) and incubated for 72 h at 37°C under 5% CO2. Three hours afte the addition of MTT the amount of formazan formed is measured spectrophotometrically at 550 nM with a Perkin Elmer HTS-7000 plate reader the test Samples are first dissolved in DMSO and then diluted with medium the final concentration of DMSO is less than 0.25%. Normal also ha the same extent of DMSO. 5-Fluorouracil (5-FU) and doxorubicin HCl are used as positive controls, and EC50 Values are calculated fro the mean Values of data from 4 wells. (Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Disclaimer: For research use only

Alternative Names

Apoptosis, Caffeic Acid Phenethyl Ester, CAPE, NF-kB, NFkB, NFκB, NF-κB, Nuclear factor-kappaB, Nuclear factor-κB, inhibit, Inhibitor, Phenylethyl Caffeate

Compound Identifiers

PubChem CID

5281787