C527

SKU: orb1308505

Description

C527

Research Area

Cell Biology, Molecular Biology, Protein Biochemistry

Key Properties

• CAS Number: 192718-06-2
• MW: 293.25
• Purity: 97.64% (May vary between batches)
• Formula: C₁₇H₈FNO₃
• SMILES: Fc1ccc(cc1)-c1nc2c(o1)C(=O)c1ccccc1C2=O
• Target: DUB
• Solubility: DMSO:1.85 mg/mL (6.31 mM)

Bioactivity

• Target IC50:
  USP1:0.88 μM
• In Vitro:
  C527 inhibits the DUB activity of the USP12/USP46 complex and other DUB enzymes in vitro. However, the IC50 of C527 for these DUB enzymes was higher in comparison with USP1/UAF1 complex. C527 has considerably less inhibitory effect on UCH-L1 and UCH-L3, a different subclass of DUB enzymes. C527 treatments causes an increase in the levels of Ub-FANCD2 and Ub-FANCI and it also lead to an increase in ubiquitinated forms of FANCD2 and FANCI, cause a decrease in homologous recombination activity, and sensitize cells to DNA damaging agents. Pretreatment of USP1/UAF1 with C527 resulted in inhibition of its enzyme activity with an IC50 of 0.88±0.03 μM. Pretreatment of cells with the C527 causes an enhancement in the cytoxicity of mitomycin C and camptothecin.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

inhibit, Inhibitor, DUBs, Deubiquitinase, C 527, C527, C-527, USP1

Compound Identifiers

PubChem CID

2307331