Buparvaquone

SKU: orb1303004

Description

Buparvaquone (BW 720C) is a hydroxynaphthoquinone antiprotozoal agent structurally analogous to parvaquone and atovaquone. It is primarily used in veterinary and research applications for studying theileriosis, demonstrating efficacy in both in vitro assays and in vivo animal models against Theileria parasites.

Research Area

Infectious Disease & Virology

Key Properties

• CAS Number: 88426-33-9
• MW: 326.43
• Purity: 99.81%
• Formula: C₂₁H₂₆O₃
• SMILES: O=C1C=2C(C(=O)C(O)=C1CC3CCC(C(C)(C)C)CC3)=CC=CC2
• Target: Parasite,Antibiotic
• Solubility: DMSO:3.27 mg/mL (10.02 mM)

Bioactivity

• In Vivo:
  Both a hydrous gel and a water-in-oil emulsion containing buparvaquone markedly decrease the cutaneous parasite load and lesion size compared to the untreated control. Administering buparvaquone (100 mg/kg) to N. caninum-infected mice, either through intraperitoneal injection or orally via gavage, effectively prevents neosporosis symptoms in a significant proportion of the subjects. Specifically, symptoms were prevented in four out of six mice in the group treated intraperitoneally and in six out of seven mice receiving oral treatment.
• In Vitro:
  Buparvaquone is significantly selective against L. (L.) infantum chagasi intracellular amastigotes, with an IC50 value of 1.5 μM. In 4-day proliferation assays, buparvaquone efficiently inhibits N. caninum tachyzoite replication(IC50=4.9 nM; IC100=100 nM). Other cutaneous species are also susceptible to buparvaquone, with IC50 values in the range 1-4 μM.
• Cell Research:
  To study whether pretreatment of host cells prior to invasion had any effect on parasite proliferation, confluent HFF grown in 6-well plates are treated with 1 μM buparvaquone in medium for 1 h or 5 h, and controls are exposed to the corresponding amounts of DMSO. Subsequently, the drug-containing medium is removed and monolayers are ished 4 times with Hank's Balanced Salt Solution, and are infected with Nc-Liv tachyzoites in 5 mL medium without any drug or solvent. After 2 days, cells are collected with a cell scraper, centrifuged, ished once more in PBS, and the pellet is stored at 20 °C prior to quantification of N. caninum proliferation by N. caninum-specific real time PCR as outlined below.
• Animal Research:
  Mice: On day 0, all mice are infected by intraperitoneal (i.p.) injection of freshly purified N. caninum tachyzoites. After 48 h, mice receive BPQ (100 mg/kg) as suspension in corn oil either by i.p. injection of a volume of 100 μl or by oral application of 100 μl by gavage. The control groups obtained the corresponding amount of the solvent only, either i.p. or orally. The treatments are performed 5 times on a daily basis. If not indicated otherwise, mice are inspected twice daily for clinical signs (ruffled coat, apathy, hind limb paralysis) until day 21 post infection (p.i.), at which time they are euthanized.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Parasite, inhibit, Inhibitor, BW 720C, Buparvaquone, Butalex, Antibiotic

Compound Identifiers

PubChem CID

71768