BSEP Antibody / Bile Salt Export Pump / ABCB11

• Catalog Number: orb1823840
• Category: Antibodies
• Description: ATP-binding cassette (ABC) transporters are an evolutionarily conserved family of proteins that catalyze the transport of molecules across extra- and intracellular membranes through the energy of ATP hydrolysis. ABC genes comprise seven subfamilies, designated ABC1, Mdr/TAP, MRP, ALD, OABP, GCN20 and White. The secretion of bile salt molecules from blood into bile is a major driving force for bile formation. Bile salt export pump (BSEP) is a member of the Mdr/TAP subfamily of ABC transporters that mediates the transport of bile acids across the hepatocyte canalicular membrane and regulates bile acid-dependent bile secretion. BSEP contains putative phosphorylation sites for protein kinase A, protein kinase C (PKC) and Ca2+-calmodulin dependent kinase II, whose regulation may be dependent on bile salt concentration.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: BSEP/7534
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG
• Immunogen: A recombinant partial protein sequence (within amino acids 600-800) from the human protein was used as the immunogen for the BSEP antibody.
• Antibody Type: Primary Antibody
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Conjugation: Unconjugated
• Formula: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Hazard Information: This BSEP antibody is available for research use only.
• UniProt ID: O95342
• Storage: Aliquot the BSEP antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human liver tissue with BSEP antibody (clone BSEP/7534). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free BSEP antibody (clone BSEP/7534) as confirmation of integrity and purity.