BS-181 hydrochloride

SKU: orb1301021

Description

BS-181 hydrochloride is a potent and selective CDK7 inhibitor (IC50 = 21 nM), demonstrating over 40-fold selectivity against CDK1, 2, 4, 5, 6, and 9. It is a valuable research tool for studying transcriptional regulation, cell cycle progression, and has been utilized in both in vitro and in vivo cancer model studies.

Research Area

Cell Biology

Key Properties

• CAS Number: 1397219-81-6
• MW: 416.99
• Purity: 99.97%
• Formula: C₂₂H₃₂N₆·HCl
• SMILES: Cl.CC(C)c1cnn2c(NCc3ccccc3)cc(NCCCCCCN)nc12
• Target: CDK
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:4 mg/mL (9.59 mM);H2O:50 mg/mL (119.91 mM);Ethanol:22 mg/mL (52.76 mM);DMSO:100 mg/mL (239.81 mM)

Bioactivity

• Target IC50:
  CDK6-CyclinD1:47 μM|CDK7:21 nM|CDK4-CyclinD1:33 μM|CDK2-CyclinE:0.88 μM|CDK9-CyclinT:4.2 μM|CDK1-CyclinB:8.1 μM|CDK5-p35 NCK:3 μM
• In Vivo:
  BS-181 is stable in vivo with a plasma elimination half-life in mice of 405 minutes after i.p. administration of 10 mg/kg. BS-181 inhibits the growth of MCF-7 xenografts in the nude mice model in a dose-dependent manner, with 25% and 50% reduction in tumor growth after 2 weeks of treatment at 10 mg/kg/day and 20 mg/kg/day, respectively without apparent toxicity.
• In Vitro:
  BS-181 is a small molecule inhibitor of CDK7 in a cell-free environment, which displays more potential activity than roscovitine with IC 50 of 510 nM. Among the CDKs and other 69 kinases from many different classes, BS-181 shows high inhibitory selectivity for CDK7, inhibits CDK2 at concentrations lower than 1 μM which being inhibited 35-fold less potently (IC50 with 880 nM) than CDK7, shows slight inhibition for CDK1, CDK4, CDK5, CDK6 and CDK9 with IC50 values higher than 3.0 μM, and only shows inhibition for several kinases from other classes at high concentrations (>10 μM). BS-181 promotes cell cycle arrest and inhibits the cancer cell growth of a range of tumor types, including breast, lung, prostate and colorectal cancer with IC50 in the range of 11.5-37 μM. In MCF-7 cells, BS-181 inhibits the phosphorylation of the CDK7 substrate RNA polymerase II COOH-terminal domain (CTD), and promotes cell cycle arrest and apoptosis to inhibit the growth of cancer cell lines.
• Cell Research:
  MCF-7 cells are exposed to BS-181 for 24 hours. For the determination of cell cycle and apoptosis, cells are stained with propidium iodide or labeled with Annexin V-FITC, then labeled cells are acquired within 1 hour, by using the RXP cytomics software on a Beckman Coulter Elite ESP, and the data are analyzed using Flow Jo v7.2.5. For the assessment of CDKs, cells are lysed and analyzed by western blotting.(Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

inhibit, Inhibitor, BS 181, BS 181 hydrochloride, BS 181 Hydrochloride, BS181, BS-181, BS-181 HCl, BS181 Hydrochloride, BS181 hydrochloride, BS-181 Hydrochloride, CDK7, CDK, Cyclin dependent kinase

Compound Identifiers

PubChem CID

49867928