BRD7552

SKU: orb1223421

Description

BRD7552 is an inducer of transcription factor PDX1, which increases insulin expression. BRD7552 increases PDX1 expression in mouse αTC cells but not βTC cells.

Key Properties

• CAS Number: 1137359-47-7
• MW: 711.63
• Purity: >98% (HPLC)
• Formula: C₃₃H₃₃N₃O₁₅
• SMILES: CCOC(=O)c1ccc(cc1)NC(=O)O[C@@H]1[C@H](O[C@@H]([C@@H]([C@H]1OC(=O)Nc1cc2c(cc1)OCO2)OC(=O)Nc1cc2c(cc1)OCO2)OC)CO
• Target: PDX1
• Solubility: DMSO : ≥ 38 mg/mL 53.40 mM

Bioactivity

• In Vitro:
  BRD7552 (0-5 μM; 5 days) induces a dose-dependent increase in PDX1 protein levels after BRD7552 treatment in PANC-1 cells. BRD7552 acts in a FOXA2-dependent manner to increase PDX1 expression in human ductal cells. Nine-day treatment of PANC-1 cells with BRD7552 (0-10 μM) causes a dose-dependent increase in insulin mRNA expression. BRD7552 induces insulin expression in PANC-1 cells. BRD7552 increases H3 acetylation and H3K4me3 and decreases H3K9me3, results that are consistent with transcriptional activation of PDX1. The epigenetic changes induced by BRD7552 are consistent with transcriptional activation, and BRD7552 acts in a FOXA2-dependent manner. Western blot analysis. Cell line: PANC-1 cells. Concentration: 1.25, 2.5, 5 μM Incubation time: 5 days. Result: Induced a dose-dependent increase in PDX1 protein levels.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

PDX1