Bovine Collagen Type VI protein

SKU: orb750380

Description

Bovine Collagen Type VI protein

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

• Tested Applications: DOT, ELISA, IHC, WB
• Application Notes:
  For use as a control or standard in indirect trapping ELISA for quantitation of antigen using a standard curve, for immunoprecipitation and for other immunological assays as optimized by the user. Specific conditions should be optimized by user.

Key Properties

• Source: Bovine
• Biological Origin: Bovine
• Target: COL6A1
• Purity: This product has been prepared from Bovine Placenta and is chromatographically and immunologically pure. This product reacts with anti-Collagen Type VI. Reaction with anti-Collagen I, II, III, IV or V is negligible (typically less than 1% cross reactivity was detected by ELISA).
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.5 M Acetic Acid
• Concentration: 1.0 mg/ml
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Collagen alpha 1 (VI) chain protein, COL6A1 protein, Bovine Collagen Type VI protein

Dot Blot results using Rabbit Anti-Collagen VI Antibody. Sample: A) Human Collagen VI (p/n orb750386), B) Bovine/Human Collagen VI (p/n orb750380). Load: 1) 1000 ng, 2) 333.33 ng, 3) 111.11 ng, 4) 37.03 ng, 5) 12.35 ng. Primary Antibody: Rabbit Anti-Collagen VI antibody at 5 µg/ml at RT for 1hr. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated (p/n orb347673) 1:40000 at RT for 30 mins. Blocking: BlockOut® Universal Blocking Buffer (p/n orb348644) at RT for 30 mins.

Quick Database Links

Gene Symbol

COL6A1