BODIPY-FL

SKU: orb1745229

Description

BODIPY-FL acid is a versatile fluorescent dye for labeling probes and primers, enabling quantitative DNA/RNA detection via fluorescence. Its derivatives, such as BODIPY-FL-labeled monoterpenes, serve as vital tools in microbiological research for detecting both Gram-positive and Gram-negative bacteria as well as pathogenic fungi in vitro.

Key Properties

• CAS Number: 165599-63-3
• MW: 292.09
• Purity: 99.05%
• Formula: C₁₄H₁₅BF₂N₂O₂
• SMILES: CC1=CC(C)=[N+]2C1=Cc1ccc(CCC(O)=O)n1[B-]2(F)F
• Target: Others
• Solubility: DMSO:90 mg/mL (308.12 mM)

Bioactivity

• In Vitro:
  I. DNA/RNA detection: 1. Primer labeling: BODIPY-FL is chemically labeled with the target DNA primer or RNA probe. After labeling, the primer can be used for real-time PCR or other nucleic acid detection methods. 2. Fluorescence detection: The fluorescence signal changes of BODIPY-FL primers or probes are observed by real-time PCR instruments or fluorescence detectors, and then the quantitative analysis of DNA/RNA is performed. 3. Quantitative analysis: The content of target DNA/RNA is quantified according to the intensity of the fluorescence signal and the reaction curve. II. Bacterial detection 1. Bacterial staining: BODIPY-FL dye is added to the bacterial sample and incubated at 37°C for 30 minutes to 1 hour. 2. Staining observation: BODIPY-FL-labeled bacteria are observed using a fluorescence microscope or flow cytometer, and real-time fluorescence imaging is performed to analyze the bacterial species and their number. 3. Identification of bacteria: Gram-positive and negative bacteria are identified based on the fluorescence characteristics of bacteria (such as color and brightness). 3. Fungal Detection 1. Fungal labeling: BODIPY-FL and fungal samples are stained and labeled by flow cytometry. 2. Microscopic observation: Fluorescent signals in samples are observed using a fluorescence microscope to confirm the fungal species.

Storage & Handling

• Storage: keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BODIPY-FL, BDP FL acid

Compound Identifiers

PubChem CID

9817511