BNIP1 Antibody

SKU: orb1247728

Description

BNIP1 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 28kDa band observed in Human Kidney lysates (calculated MW of 26.1kDa according to NP_001196.1; NP_053581.1; NP_053582.1; NP_053583.1). Recommended concentration: 0.5-1.5µg/mL. An additional band of unknown identity was also consistently observed at 48kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-DLLRQRKTTKESLAQ
• Target: BNIP1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BNIP1, BCL2/adenovirus E1B 19kDa interacting protein 1, NIP1, SEC20, TRG-8, BCL2/adenovirus E1B 19kD interacting protein 1, BCL2/adenovirus E1B 19kD-interacting protein 1

orb1247728 (0.5 ug/ml) staining of Human Kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

BNIP1

UniProt

Q12981

RefSeq (Protein):NP_001196.1, NP_053583.1, NP_053581.1, NP_053582.1