Bilobetin

SKU: orb1222122

Description

Bilobetin ameliorates insulin resistance by PKA-mediated phosphorylation of PPARα in rats fed a high-fat diet.

Key Properties

• CAS Number: 521-32-4
• MW: 552.49
• Purity: >98% (HPLC)
• Formula: C₃₁H₂₀O₁₀
• SMILES: Oc1cc(O)cc2OC(=CC(=O)c12)c6cc(c5c(O)cc(O)c3c5OC(=CC3=O)c4ccc(O)cc4)c(OC)cc6
• Target: PPARα
• Solubility: In Vitro: DMSO : 250 mg/mL (452.51 mM)

Bioactivity

• In Vivo:
  Animal model: rats fed a high-fat diet. Dosage: 12 mg/kg/day. Administration: i.p. , 4 or 14 days after mice were fed a HFD for 8 weeks. Result: Increased glucose infusion rate (GIR, P < 0.05) and reduced EGP (P < 0.05) in basal and clamp states. Reduced total TG (P < 0.01) and VLDL-TG (P < 0.01). Enhances hepatic Intralipid-TG uptake. Reduced the total amount of lipid level in the liver and muscle. Promoted the phosphorylation of PPARα and the translocation of PPARα from the cytoplasm to the nucleus in rats liver.
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• In Vitro:
  Cell Viability Assay Cell line: Huh7 and HepG2 cells. Concentration: 0, 2.5, 5, 10, 20, and 40 μM Incubation time: 24/48/72 h. Result: Inhibited cell proliferation in a dose dependent manner, with IC50s of 18.28 μM at 48 h in Huh7 cells, and 19 μM at 72 h in HepG2 cells. Apoptosis Analysis Cell line: Huh7 and HepG2 cells. Concentration: 0, 2.5, 5, 10, 20 μM Incubation time: 24/48 h. Result: Showed the total apoptotic cell rate of 2.2, 2.2, 1.9, and 10.6% for 24 h (Huh7 cells) at 0, 5, 10, and 20 μM, and 1.7, 1.5, 7.5, and 22.5% for 48 h (Huh7 cells) at 0, 5, 10, and 20 μM. Showed the total apoptotic cell rate of 1.1, 1.2, 1.6, and 3.0% for 24 h (Huh7 cells) at 0, 5, 10, and 20 μM, and 1.5, 1.1, 3.2, and 14.2% for 48 h (Huh7 cells) at 0, 5, 10, and 20 μM, respectively.
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Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

PPARα