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BAFF/TNFSF13B Antibody

SKU: orb1539112

Description

BAFF/TNFSF13B Antibody

Images & Validation

Tested ApplicationsELISA, IHC, IHC-P, WB
Dilution RangeELISA (5 - 6 µg/ml), IHC, IHC-P (10 µg/ml), WB (0.25 - 0.5 µg/ml)
ReactivityHuman
Application Notes
Resuspension: Reconstitute with sterile water. Further information: This in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 10 ug/ml is recommended. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 5-6 ug/ml of this antibody will detect at least 1000 pg/ml of recombinant human BAFF when used with biotinylated antigen affinity purified anti-human BAFF as the detection antibody at a concentration of approximately 0.25-0.50 ug/ml. Western Blot: To detect hBAFF by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBAFF is 2-4 ng/lane, under non-reducing conditions.

Key Properties

HostMouse
ClonalityMonoclonal
IsotypeIgG1,k
ImmunogenE.coli derived recombinant Human BAFF.
TargetBAFF / TNFSF13B
PurificationProtein G purified
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLyophilized from PBS
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

TNFSF13B, B-cell-activating factor, BAFF, ApoL related ligand TALL-1, B-lymphocyte stimulator, CD257, BLYS, Delta4 BAFF, Delta BAFF, TALL-1, TALL1, ZTNF4, THANK, B lymphocyte stimulator, CD257 antigen, TNFSF20

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Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

BAFF/TNFSF13B Antibody (orb1539112)

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500 μg
$ 640.00
DispatchUsually dispatched within 2-3 weeks
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