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Catalog Number | orb1539112 |
---|---|
Category | Antibodies |
Description | BAFF/TNFSF13B Antibody |
Target | BAFF / TNFSF13B |
Clonality | Monoclonal |
Species/Host | Mouse |
Isotype | IgG1,k |
Conjugation | Unconjugated |
Reactivity | Human |
Buffer/Preservatives | Lyophilized from PBS. Lyophilized from PBS |
Purification | Protein G purified |
Immunogen | E.coli derived recombinant Human BAFF. |
Tested applications | ELISA, IHC, IHC-P, WB |
Dilution range | ELISA (5 - 6 µg/ml), IHC, IHC-P (10 µg/ml), WB (0.25 - 0.5 µg/ml) |
Application notes | Resuspension: Reconstitute with sterile water. Further information: Immunohistochemistry: LS-B8201 in an antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 10 ug/ml is recommended. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 5-6 ug/ml of this antibody will detect at least 1000 pg/ml of recombinant human BAFF when used with biotinylated antigen affinity purified anti-human BAFF (LS-C104386) as the detection antibody at a concentration of approximately 0.25-0.50 ug/ml. Western Blot: To detect hBAFF by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBAFF is 2-4 ng/lane, under non-reducing conditions. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | TNFSF13B, B-cell-activating factor, BAFF, ApoL rel Read more... |
Note | For research use only |
ELISA, FC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |