BACH2 Antibody (C-term)

SKU: orb1939151

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cell Biology, Immunology & Inflammation, Metabolism Research

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: WB: 1:2000, FC: 1:25, FC: 1:10~50
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB21865
• Target: This BACH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 815-841 amino acids from the C-terminal region of human BACH2.
• Molecular Weight: 92537 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Transcription regulator protein BACH2, BTB and CNC homolog 2, BACH2

All lanes: Anti-BACH2 Antibody (C-term) at 1:2000 dilution. Lane 1: 293T/17 whole cell lysate. Lane 2: Daudi whole cell lysate. Lane 3: K562 whole cell lysate. Lane 4: Ramos whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 93 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

BACH2 Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Overlay histogram showing Ramos cells (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

Quick Database Links

Gene Symbol

This BACH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 815-841 amino acids from the C-terminal region of human BACH2.

UniProt

Q9BYV9

RefSeq (Protein):NP_068585.1, NP_001164265.1