AUDA

SKU: orb1299402

Description

AUDA is a potent soluble epoxide hydrolase (sEH) inhibitor, exhibiting IC50 values of 18 nM (mouse) and 69 nM (human). It is a valuable research tool for studying the role of epoxyeicosatrienoic acids in inflammation, hypertension, and pain in both cellular and animal models.

Research Area

Metabolism Research

Key Properties

• CAS Number: 479413-70-2
• MW: 392.58
• Purity: ≥95%
• Formula: C₂₃H₄₀N₂O₃
• SMILES: N(C(NCCCCCCCCCCCC(O)=O)=O)C12CC3CC(C1)CC(C2)C3
• Target: Epoxide Hydrolase
• Solubility: DMSO:30 mg/mL (76.42 mM)

Bioactivity

• Target IC50:
  sEH:18 nM (mouse)|sEH:69 nM (human)
• In Vivo:
  AUDA increased the proliferation, migration, adhesion and tube formation ability of HCAECs in a dose-dependent manner. Furthermore, in the mouse model of KD, AUDA reduced the protein expression of MMP-9, IL-1β and TNF-α, indicating that AUDA may alleviate inflammatory reactions in the coronary arteries of KD model mice.
• In Vitro:
  AUDA significantly inhibited PDGF-induced rat VSMC proliferation, which coincided with Pin1 suppression and heme oxygenase-1 (HO-1) upregulation. However, exogenous 8,9-EET, 11,12-EET, and 14,15-EET treatments did not alter Pin1 or HO-1 levels and had little effect on the proliferation of rat VSMCs. On the other hand, AUDA enhanced the PDGF-stimulated cell migration of rat VSMCs. Furthermore, AUDA-induced activation of cyclooxygenase-2 (COX-2) and subsequent thromboxane A2 (TXA ) production were required for the enhanced migration.
• Cell Research:
  Cell Line:Vascular smooth muscle cell (VSMC).Concentration:0.3, 1, 3, 10 μg/mL. Incubation Time:48 hours
• Animal Research:
  AUDA (i.p.; 10 mg/kg; 14 days) reduces TNF-α, MMP-9 and IL-1β expression levels.Animal Model:Male (wild-type). C57BL/6 mice (age, 4-6 weeks; weight, 18-20 g).Dosage:10 mg/kg.Administration:i.p.; 14 days

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti-inflammatory, AUDA, activity, Inhibitor, inhibit, Epoxide Hydrolase, epoxide, EpoxideHydrolase, hydrolase, VSMC, soluble, sEH

Compound Identifiers

PubChem CID

10069117