ATP5J Antibody (Center)

• Catalog Number: orb1937457
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB31956
• Tested applications: IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Dilution range: IF: 1:10~50, WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 12588 Da
• Target: This ATP5J antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 28-56 amino acids from the Central region of human ATP5J.
• UniProt ID: P18859
• NCBI: NP_001003703.1, NP_001676.2, NP_001003701.1, NP_001003696.1, NP_001003697.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: ATP synthase-coupling factor 6, mitochondrial, ATP
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

ATP5J Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of ATP5J Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

ATP5J Antibody (Center) western blot analysis in mouse stomach tissue lysates (35 ug/lane). This demonstrates the ATP5J antibody detected the ATP5J protein (arrow).

Fluorescent confocal image of U251 cell stained with ATP5J Antibody (Center). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ATP5J primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). ATP5J immunoreactivity is localized to Mitochondria significantly.

Western blot analysis of lysate from rat heart tissue lysate, using ATP5J Antibody (Center). diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary Antibody. Lysate at 20 ug.