ATG13 Antibody

SKU: orb345691

Description

ATG13 antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:220,000-1:250,000, WB: 1:1000
• Reactivity: Human
• Application Notes:
  This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 56.6 kDa in size corresponding to human ATG13 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to the S318 region of ATG13.
• Purity: This affinity-purified antibody is directed against human ATG13 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with ATG13 from human based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.24 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-ATG13 Antibody, ATG-13, ATG 13, Autophagy-related protein 13, KIAA0652

Western blot using Biorbyt's affinity purified anti-ATG13 antibody shows detection of ATG13 in 293T cells engineered to coexpress Ulk1 and Atg13 (Ulk1 + Atg13), right lane, but not in the left lane in which was loaded kinase-dead hypophosphorylated Ulk1-K46A mutaNT+ ATG13. Detection is demonstrated at approximately 57 kDa. The antibody was purified and resolved by SDS-PAGE, then transferred to nitrocellulose membrane. The membrane was blocked with 5% Blotto (p/n orb348624) and probed with the primary antibody at 1 µg/ml overnight at 4°C. After washing, the membrane was probed with Goat Anti-Rabbit HRP secondary 1:5000 in detection buffer (p/n orb348637) for 45 minutes at room temperature.

Quick Database Links

UniProt

O75143

RefSeq (Protein):NP_001136145.1