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Item 1 of 2
ARNT Antibody (Center V528)
Catalog Number: orb1935930
| Catalog Number | orb1935930 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB00060 |
| Tested applications | IF, WB |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | IF: 1:10~50, WB: 1:1000 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Conjugation | Unconjugated |
| MW | 86636 Da |
| Target | This ARNT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 513-544 amino acids from the Central region of human ARNT. |
| UniProt ID | P27540 |
| NCBI | NP_001659.1, NP_848514.1, NP_001184254.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Aryl hydrocarbon receptor nuclear translocator, AR Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
ARNT Antibody (V528) western blot analysis in NCI-H460 cell line lysates (35 ug/lane). This demonstrates the ARNT antibody detected the ARNT protein (arrow).
Fluorescent image of A549 cell stained with hARNT-V528. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with hARNT primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). hARNT immunoreactivity is localized to Nucleus significantly.
