ARGBP2 Antibody (N-term)

• Catalog Number: orb1929665
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB19714
• Tested applications: FC, IF, IHC-P, WB
• Predicted Reactivity: Mouse, Porcine, Rat
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: IF: 1:10~50, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 124108 Da
• Target: This ARGBP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 175-204 amino acids from the N-terminal region of human ARGBP2.
• UniProt ID: O94875
• NCBI: NP_001139145.1, NP_003594.3, NP_001139143.1, NP_001139142.1, NP_001257700.1, NP_001139146.1, NP_066547.1, NP_001139144.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Sorbin and SH3 domain-containing protein 2, Arg/Ab
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of MDA-231 cells using ARGBP2 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Fluorescent image of U251 cell stained with ARGBP2 Antibody (N-term).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ARGBP2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C).ARGBP2 immunoreactivity is localized to Nucleus significantly.

Formalin-fixed and paraffin-embedded human colon carcinoma reacted with ARGBP2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of ARGBP2 Antibody (N-term) in MDA-MB231 cell line lysates (35 ug/lane). ARGBP2 (arrow) was detected using the purified Pab.