Ara-G

SKU: orb1303886

Description

Ara-G is a deoxyguanosine analog metabolized to araGTP, selectively inhibiting DNA synthesis in T-cell lineages. It demonstrates potent in vitro toxicity against T-lymphoblastoid cell lines and primary T-ALL cells, supporting its research use in leukemia studies and T-cell biology.

Research Area

Cell Biology, Molecular Biology

Key Properties

• CAS Number: 38819-10-2
• MW: 283.24
• Purity: 99.93% (May vary between batches)
• Formula: C₁₀H₁₃N₅O₅
• SMILES: Nc1nc2n(cnc2c(=O)[nH]1)[C@@H]1O[C@H](CO)[C@@H](O)[C@@H]1O
• Target: Nucleoside Antimetabolite/Analog,Others
• Solubility: Ethanol:Partially Soluble;DMF:3 mg/mL (10.59 mM);DMSO:55 mg/mL (194.18 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (7.06 mM);PBS (pH 7.2):0.3 mg/mL (1.06 mM)

Bioactivity

• In Vitro:
  Ara-G accumulates in T lymphoblasts and malignant T-lymphoid cells, where it is phosphorylated to produce ara-GTP and incorporated into the DNA.3,1 Ara-G inhibits DNA replication by 92% after 30 minutes when used at a concentration of 50 μM in CEM cells, which are used as a model for human T lymphoblasts.1 It also halts the cell cycle at the sub-G1 phase and induces apoptosis in CEM cells.3 Syngeneic bone marrow containing 6C3HED tumor cells treated with ara-G (100 mM) ex vivo prior to transplantation increases survival of lethally irradiated mice and induces reconstitution of lymphoid, myeloid, and erythroid cell linages.4

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Analog, Ara G, AraG, Ara-G, Nucleoside Antimetabolite/Analog, Nucleoside Antimetabolite, NucleosideAntimetabolite

Compound Identifiers

PubChem CID

135499520