APTS

SKU: orb1303947

Description

8-Aminopyrene-1,3,6-trisulfonic acid sodium salt is an anionic fluorescent dye with excitation/emission at 425/503 nm. It serves as a key reporter for glucose and saccharide sensing in vitro and can be immobilized onto aldehyde-functionalized surfaces via reductive amination without significant fluorescence loss.

Key Properties

• CAS Number: 196504-57-1
• MW: 523.4
• Purity: 99.79% (May vary between batches)
• Formula: C₁₆H₈NNa₃O₉S₃
• SMILES: O=S(C1=C(C2=C34)C=CC4=C(N)C=C(S(=O)([O-])=O)C3=CC=C2C(S(=O)([O-])=O)=C1)([O-])=O.[Na+].[Na+].[Na+]
• Target: Others
• Solubility: PBS (pH 7.2):10 mg/mL (19.11 mM);DMSO:2 mg/mL (3.82 mM);DMF:0.2 mg/mL (0.38 mM)

Bioactivity

• In Vitro:
  APTS labeling of N-glycans Procedure: 1. Use sodium cyanoborohydride (NaBH3CN) as a reducing agent. Dissolve a mixture of N-glycans (∼100 μg) and isomaltose (25 pmol) in a 0.2 mL-PCR thin-wall tube. 2. Dissolve 5 μL of 50 mM APTS in 500 mM citric acid. 3. Add 5 μL of 1.0 M NaBH3 to the sample mixture and incubate the mixture at 55°C for 50 min. Quench the reaction with 140 μL of cold distilled water (total volume, 150 μL) and purify. 4. Purify the APTS-labeled N-glycans by passing through IPAE and HILIC-SPE spin columns.

Storage & Handling

• Storage: keep away from direct sunlight,keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anionic, APTS, 8 Aminopyrene 1,3,6 trisulfonic Acid sodium salt, 8-Aminopyrene-1,3,6-trisulfonic Acid sodium, 8Aminopyrene1,3,6trisulfonic Acid sodium salt, 8-Aminopyrene-1,3,6-trisulfonic acid trisodium, Inhibitor, inhibit, water-soluble

Compound Identifiers

PubChem CID

9849652