ANXA2 Antibody, KO Validated

• Catalog Number: orb1274524
• Category: Antibodies
• Description: ANXA2 Antibody, KO Validated
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-339 of human ANXA2 (NP_004030.1).
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 38kDa
• Target: ANXA2
• UniProt ID: P07355
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: ANXA2, P36, ANX2, LIP2, LPC2, CAL1H, LPC2D, ANX2L4
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:20 - 1:50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using ANXA2 Antibody (orb1274524) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Western blot analysis of extracts from normal (control) and ANXA2 knockout (KO) HeLa cells, using ANXA2 antibody (orb1274524) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Immunohistochemistry of paraffin-embedded rat heart using ANXA2 antibody (orb1274524) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse liver using ANXA2 antibody (orb1274524) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse heart using ANXA2 antibody (orb1274524) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of A549 cells using ANXA2 antibody (orb1274524). Blue: DAPI for nuclear staining.