Anti-TCR beta [IARC307]

SKU: orb2645831

Description

Anti-TCR beta [IARC307]

Research Area

Disease Biomarkers, Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, FC, IF
• Reactivity: Human

Key Properties

• Host: Human
• Clonality: Monoclonal
• Isotype: IgM
• Clone No.: IARC307
• Immunogen: Derived from normal B cells in peripheral blood of a Burkitt’s lymphoma patient. Screened more than 60 culture supernatants from lymphoblastoid cell lines of normal origin, Epstein-Barr virus (EBV)-carrying Burkitt lymphomas, EBV-negative Burkitt lymphomas and EBV-converted sublines of original EBV-negative Burkitt lymphoma human B-cell lines for their capacity to bind the synthetic peptide autoantigens. One lymphoblastoid B-cell line was identified, IARC307, that produces IgM kappa antibodies which specifically react with the CDR1 peptide corresponding to human Vbeta8.1 gene sequence.
• Target: TCR beta
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

IARC-307; TCR-beta; T cell receptor; T-cell receptor; T cell receptor beta chain; TCRB; TRB; TCB1; Ti; TCR V beta; IARC/307; IARC 307

Flow cytometry using the Anti-TCR beta antibody IARC307. Paraformaldehyde fixed Jurkat cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of IARC307 (orb2645830, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of Jurkat cells with anti-TCR beta IARC307. Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of IARC307 (orb2645830) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb2645830, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Quick Database Links

Gene Symbol

TCR beta

UniProt

A0A5B9, P01850