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Anti-PKA-RIB (Protein Kinase-A Regulatory Subunit Ib)

SKU: orb1138130

Description

Rabbit polyclonal antibody to PKA-RIB (Protein Kinase-A Regulatory Subunit Ib)

Images & Validation

Tested ApplicationsWB
Dilution RangeRecommended dilutions WB: 1/1,000 when using colorimetric substrates such as BCIP/NBT and 1/5,000 for chemiluminescent substrates. Higher concentration of antibody may be needed for non-human samples. Predicted molecular weight: 43 kDa. This antibody identifies bands at 55 kD and 53 kD when used against the reduced protein as well as activation/breakdown products_recover. The antibody can recognize native or reduced PKARIb. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
ReactivityHuman, Mouse, Other, Rat
Application Notes
Recommended dilutions WB: 1/1,000 when using colorimetric substrates such as BCIP/NBT and 1/5,000 for chemiluminescent substrates. Higher concentration of antibody may be needed for non-human samples. Predicted molecular weight: 43 kDa. This antibody identifies bands at 55 kD and 53 kD when used against the reduced protein as well as activation/breakdown products_recover. The antibody can recognize native or reduced PKARIb. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenSynthetic peptide of Rat Protein Kinase A regulatory subunit I beta.
PurityImmunogen Affinity purified
PurificationImmunogen Affinity purified
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservativesphosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration1ug/ul
Expiration Date12 months from date of receipt.
DisclaimerFor research use only
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Protocol Information

WB
Western Blot (IB, immunoblot)
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Anti-PKA-RIB (Protein Kinase-A Regulatory Subunit Ib) (orb1138130)

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100 μg
$ 1,540.00
DispatchBackorder, please inquire.
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