Anti-p16 INK4a Antibody

• Catalog Number: orb666246
• Category: Antibodies
• Description: Rabbit polyclonal antibody to p16 INK4a
• Target: CDKN2A
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide of human p16 INK4a. The exact sequence is proprietary.
• UniProt ID: P42771, Q9R0Z3, P51480
• Tested applications: IF, IH, WB
• Dilution range: WB: 1-500-2000, IF: 1-50-200
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-CDKN2 antibody, anti-MTS1 antibody, anti-Cycl
• Note: For research use only
• Entrez: 12578, 1029, 25163

Western blot analysis of p16 INK4a expression in HeLa (A), MCF7 (B) whole cell lysates. (Predicted band size: 8; 11; 12; 13; 16; 17 kD; Observed band size: 17 kD)

Immunohistochemical analysis of p16 INK4a staining in mouse testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of p16 INK4a staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.