Anti-MHC I (RT1A) [OX-18]

SKU: orb2645721

Description

Anti-MHC I (RT1A) [OX-18]

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, FC, IHC-Fr, IP
• Reactivity: Rat

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: OX-18
• Immunogen: OX-18 was prepared by immunizing BALB/c mice with rat spleen glycoproteins of the size of Ia antigens which has been depleted of Ia antigens using an OX-6 affinity column.
• Target: MHC I (RT1A)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

RT-1A; RT-1A histocompatibility antigen; non-polymorphic determinant of rat MHC class I; MHC class I; OX18; MRC OX18; MRC OX-18

Flow cytometry using the Anti-MHC I (RT1A) antibody OX-18. Paraformaldehyde fixed rat splenocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of OX-18 (orb2645720, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed rat splenocytes with anti-MHC I (RT1A) antibody OX-18. Immunofluorescence analysis of paraformaldehyde fixed rat splenocytes on Shi-fix™ cover-slips stained with the chimeric rabbit IgG version of OX-18 (orb2645720) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb2645720, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using anti-MHC I (RT1A) antibody OX-18. Rat lymph node tissue lysates (35 µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of OX-18 (orb2645720) at 0.01 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Quick Database Links

Gene Symbol

MHC I (RT1A)

UniProt

P16391