KANK2 Antibody

SKU: orb2649527

Description

Rabbit polyclonal antibody to KANK2

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution range: WB: WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200), IF: WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Clone No.: KANK2
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Alternative Names

ANKRD25; KIAA1518; MXRA3; SIP; KN motif and ankyrin repeat domain-containing protein 2; Ankyrin repeat domain-containing protein 25; Matrix-remodeling-associated protein 3; SRC-1-interacting protein; SIP; SRC-interacting protein; SRC1-interacting protein

Western blot analysis of KANK2 expression in A375 (A), SKOVCAR3 (B) whole cell lysates.

Immunohistochemical analysis of KANK2 staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of KANK2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Quick Database Links

UniProt

Q63ZY3