Anti-HLA-DR [BAG 14D6]

SKU: orb2645629

Description

Anti-HLA-DR [BAG 14D6]

Research Area

Immunology & Inflammation

Images & Validation

• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: BAG 14D6
• Immunogen: This antibody was raised by immunizing mice against HLA-DR.
• Target: HLA-DR
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD74; HLA class II histocompatibility antigen gamma chain; HLA-DR antigens-associated invariant chain; Ia antigen-associated invariant chain; Ii; p33

Flow cytometry using anti-HLA-DR antibody BAG 14D6. Daudi cells were stained with the anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of BAG 14D6 (orb2645628, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and the cells were analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of Daudi cells with anti-HLA-DR antibody BAG 14D6. Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of BAG 14D6 (orb2645628) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, orb2645628, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Quick Database Links

Gene Symbol

HLA-DR

UniProt

P04233