Anti-GITR [YGITR 860.103.5]

SKU: orb613834

Description

Rabbit monoclonal antibody to GITR

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FC
• Reactivity: Mouse

Key Properties

• Host: Rat
• Clonality: Monoclonal
• Isotype: Rat IgG
• Clone No.: YGITR 860.103.5
• Immunogen: Mouse GITR.
• Target: GITR
• Purity: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD357; Tumor necrosis factor receptor superfamily member 18; TNFRSF18; AITR; GITR-D; activation-inducible TNFR family receptor; AITR; glucocorticoid-induced tumor necrosis factor receptor

Flow-cytometry using anti-GITR antibody YGITR860.103.5 Mouse spleenocyts were stained with an isotype control (orb256390, Orange) or the rabbit-chimeric version of YGITR860.103.5 (orb613834) at a concentration of 1 µg/ml for 30 mins at RT without (Blue) and with (Grey) stimulation of the leukocytes with immobilized anti-CD3 and anti-CD28 antibodies. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed mouse splenocytes with anti-GITR (Tumor necrosis factor ligand superfamily member 18) antibody YGITR 860.103.5. Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on Shi-fix™ coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version of YGITR 860.103.5 (orb613834) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing membrane and some cytoplasmic staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb613834, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor® 488 secondary antibody.

Quick Database Links

Gene Symbol

GITR