Anti-Fibrin beta-chain [59D8]

• Catalog Number: orb2645810
• Category: Antibodies
• Description: Anti-Fibrin beta-chain [59D8]
• Target: Fibrin beta-chain
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Immunogen: The original antibody was generated by using a synthetic heptapeptide from the amino terminus of the beta chain in human fibrin as an immunogen.
• UniProt ID: P02675
• Tested applications: ELISA
• Clone Number: 59D8
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: FGB; factor Ia; factor-1a; fibrin; fibrin β chain;
• Note: For research use only

Immunofluorescence staining of HepG2 cells with anti-Fibrin beta-chain antibody 59D8. Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 59D8 (orb2645810) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, orb2645810, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western blot using anti-Fibrin beta-chain antibody 59D8. Human liver (A), placenta (B), and serum (C) tissue lysates (35 µg protein in RIPA buffer) were resolved via SDS-PAGE, and the subsequent blots were probed with the chimeric rabbit version of 59D8 (orb2645810) at 0.01 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.