Anti-Ferritin [F11 (HSF102)]

• Catalog Number: orb2645773
• Category: Antibodies
• Description: Anti-Ferritin [F11 (HSF102)]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: F11 (HSF102)
• Tested applications: ELISA
• Reactivity: Human
• Isotype: IgG2a
• Immunogen: The original antibody was generated by immunizing female Balb/c mice with ferritin extracted from human spleen cells.
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• Target: Ferritin
• UniProt ID: Q8N4E7
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: FTMT; FTL; FTH1; Ferritin mitochondrial; B8
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using anti-Ferritin antibody F11 (HSF102). Paraformaldehyde fixed MCF7 cells permeabilized with 0.5% Triton were stained with the anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of F11 (HSF102) (orb2645772, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000, and the cells were analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of MCF7 cells with anti-Ferritin antibody F11. Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton, on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of F11 (orb2645772) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic/mitochondrial staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, orb2645772, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.