EGFR (pY869) Antibody

SKU: orb2649545

Description

Rabbit polyclonal antibody to EGFR (pY869)

Research Area

Cell Biology

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200), IF: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Clone No.: EGFR
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ERBB; ERBB1; HER1; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

Western blot analysis of EGFR (pY869) expression in Hela (A) whole cell lysates.

Immunofluorescent analysis of EGFR (pY869) staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Quick Database Links

UniProt

P00533