EGFR (pY869) Antibody

• Catalog Number: orb2649545
• Category: Antibodies
• Description: Rabbit polyclonal antibody to EGFR (pY869)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human
• UniProt ID: P00533
• Tested applications: IF, WB
• Dilution range: WB: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200), IF: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)
• Clone Number: EGFR
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ERBB; ERBB1; HER1; Epidermal growth factor recepto
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of EGFR (pY869) expression in Hela (A) whole cell lysates.

Immunofluorescent analysis of EGFR (pY869) staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.