Anti-DDX3X Antibody

• Catalog Number: orb393265
• Category: Antibodies
• Description: Rabbit polyclonal antibody to DDX3X.
• Target: DDX3X
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human DDX3X. The exact sequence is proprietary.
• UniProt ID: O00571
• Tested applications: IF, IH, WB
• Dilution range: WB: 1:500:1000, IHC-P: 1:100:200, IF/ICC: 1:100:500
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: DBX; DDX3; ATP-dependent RNA helicase DDX3X; DEAD
• Note: For research use only
• Entrez: 1654

Western blot analysis of DDX3X expression in HepG2 (A), Hela (B) whole cell lysates. (Predicted band size: 71; 73 kD; Observed band size: 75 kD)

Immunohistochemical analysis of DDX3X staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of DDX3X staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).